Extended Data Fig. 6: EEG setup and recordings, click-iT chemistry and immunohistochemical analysis of factor V and troponin I.

a, Placement of EEG electrodes on the porcine scalp. b, Representative snapshot of the EEG recordings after administration of anaesthesia and before the induction of cardiac arrest by ventricular fibrillation. c, Representative snapshot of the EEG recordings immediately following the ventricular fibrillation. d, Representative snapshot of the EEG during ECMO intervention at around 2h of perfusion protocol. e, Representative snapshot of the EEG during OrganEx intervention at around 2h of perfusion protocol, showing a light pulsatile artefact. f, g, Representative snapshot of the EEG recordings following contrast injection at 3h in ECMO and OrganEx animals, respectively. OrganEx EEG snapshot is consistent with a possible muscle-movement artefact. GND, ground electrode; REF, reference electrode. h, i, Representative confocal images of AHA through Click-iT chemistry in newly synthesized proteins with DAPI nuclear stain in the long-term organotypic hippocampal slice culture in CA3 (h) and DG (i) subregions. j, k, Relative intensity of nascent protein around nuclei in hippocampal CA3 (j) and DG (k) region showing comparable protein synthesis between OrganEx and 0h WIT up to 14 days in culture. n = 3-5. l, Representative confocal images of immunofluorescent staining for troponin I in the heart. m, Quantification of troponin I immunolabeling signal intensity in heart. A decreased trend in immunolabeling intensity was observed with ischaemia time and a significant decrease in immunolabeling intensity in ECMO compared to the OrganEx group. n = 3. n, Representative confocal images of immunofluorescent staining for factor V in liver. o, Quantification of factor V immunolabeling signal intensity in liver follows a similar pattern seen with other organs with comparable results between 0h WIT, 1h WIT, and OrganEx group and a significant decrease in 7h WIT and ECMO groups. n = 3. Scale bars, 50 μm. Data presented are mean ± s.e.m. For more detailed information on statistics and reproducibility, see methods. *P < 0.05, **P < 0.01. AU, arbitrary units.