Extended Data Fig. 7: Quality control of snRNA-seq data in healthy and varying ischaemic conditions in the hippocampus, heart, liver, and kidney.

Through transcriptomic integration and iterative clustering, we generated a taxonomy of t-types in healthy organs and brain, heart, liver, and kidney that experienced ischaemia (1h WIT, 7h WIT, ECMO and OrganEx), representing presumptive major cell types across organs of interest. These major t-types were further subdivided into high-resolution subclusters that were transcriptomically comparable to publicly available human single-cell datasets and that were marked by distinct expression profiles (c-f)^51,52,53,54. a, Bar plot showing the number of cells/nuclei across organs and biological replicates. b, Violin plot showing the distribution of the number of unique molecular identifiers – UMIs (upper panel) and genes (lower panel) detected across all biological replicates. c-f, respective analyses of snRNA-seq in the hippocampus (c), heart (d), liver (e), and kidney (f). The left upper corner depicts detailed UMAP layout showing all t-types in the respective organs. The right side depicts the expression of top t-type markers. The left lower corner depicts transcriptomic correlation between the t-type taxonomy defined in this study and that of previous human and mouse datasets^51,52,53,54. c., cells; LSECs, liver sinusoidal endothelial cells; end., endothelium; prog., progenitor; perit., peritubular; TAL, thick ascending limb; dend., dendritic; CNT, connecting tubule.