Extended Data Fig. 11: Liver single-nucleus transcriptome analysis comparing OrganEx to other experimental conditions.

a, AUC scores of the Augur cell type prioritization between OrganEx and other groups. b, Volcano plot showing DEGs in hepatocytes between OrganEx and 0h WIT, 1h WIT, 7h WIT, and ECMO. c, Trajectories of liver hepatocytes. Colour indicates different experimental groups. d, Sankey plot showing perfusate components and violin plots showing their effects on hepatocytes between the OrganEx and ECMO. e, Hierarchical clustering of the top DEGs across experimental groups and derived functional gene modules (upper left). Eigengene average expression trends exhibit distinct trends between ECMO and OrganEx groups (lower left) of modules whose enriched GO terms are predominantly related to cellular function or cell death (right) (Supplementary Table 5). f, Expression of the genes involved in cell-death pathways in hepatocytes. g, Gene expression enrichment of the genes involved in cell-death pathways in hepatocytes. h, Stacked bar plot showing relative information flow for each signalling pathway across experimental group pairs. Significant signalling pathways were ranked based on differences in the overall information flow within the inferred networks between OrganEx and 0h WIT, 1h WIT, 7h WIT, and ECMO. Genes important in inflammation are highlighted grey. i, Overall signalling patterns across all experimental conditions. Genes important in inflammation are highlighted grey. Necro-1, necrostatin-1; Mino, minocycline; DEXA, dexamethasone; Met. B, methylene blue; GEE, Glutathione Ethyl Ester. *P < 0.05, **P < 0.01, ***P < 0.001, NS: not significant.