Extended Data Fig. 3: Representative images of H&E staining across assessed peripheral organs and kidney periodic acid-Schiff (PAS) staining and immunolabeling for HACVR1 and Ki-67.

a, Representative images of the H&E staining in heart, kidney, liver, lungs, and pancreas. Arrows point to nuclear damage, asterisks point to disrupted tissue integrity, empty arrowheads point to haemorrhage, full arrowheads point to cell vacuolization, double arrows point to tissue oedema. b, c, H&E histopathological scores in lungs (b) and pancreas (c). d, Representative images of PAS staining of the kidney. Arrows point to disrupted brush border, full arrowheads point to the presence of casts, asterisks point to tubular dilation, double arrows point to the Bowman space dilation. e, Kidney PAS histopathological damage score. n = 5. f, h, Representative confocal images of immunofluorescent staining for HAVCR1 and Ki-67 in kidney, respectively. g, Quantification of HAVCR1 immunolabeling signal intensity. i, j, Quantification of the kidney Ki-67 positive staining. HACVR1 and Ki-67 immunolabeling quantification results follow a similar pattern seen with other organs with comparable results between 0h WIT and OrganEx group and significant decrease in the 7h WIT and ECMO groups. n = 3. Scale bars,100 μm. Data presented are mean ± s.e.m. One-way ANOVA with post-hoc Dunnett’s adjustments was performed. For more detailed information on statistics and reproducibility, see methods. *P < 0.05, **P < 0.01.