Extended Data Fig. 3: Intermolecular teixobactin – teixobactin interactions seen by ssNMR.
From: Teixobactin kills bacteria by a two-pronged attack on the cell envelope
Solid-state NMR 13C-13C PARISxy spectra of the 13C,15N-teixobactin – Lipid II complex in membranes show several cross-peaks (in red) that cannot be intramolecular contacts because this would require magnetization transfer over more than 10 Å. Rather, these cross-peaks correspond to intermolecular contacts between different teixobactin molecules and agree with the formation of antiparallel β-sheets. Spectra were acquired at 950 MHz and 18 kHz MAS using 50 (purple) and 600 (cyan) ms CC magnetization transfer. a) and b) show zooms into spectral Cα-Cα and Cα-Cβ regions, while c) shows long-range interactions with the aromatic sidechain of Phe1.
