Extended Data Fig. 6: The impacts of high glucose feeding in glycolytic metabolism and tumour microenvironment.
From: Brown-fat-mediated tumour suppression by cold-altered global metabolism
a, Tumour growth in subcutaneous murine CRC-bearing mice fed with various doses of glucose (n = 6 mice per group). b, Blood glucose levels of fasting (left) and non-fasting (right) mice receiving vehicle and 15% glucose solution (n = 6 mice per group). c-j, Immunofluorescence staining and quantifications of CA9+ hypoxic area, Ki-67+ proliferating cells, CD31+ microvessels, and Cl-Casp3+ apoptotic cells in HG-treated CRC (c, d), HG-treated melanoma (f, g), and HG-treated PDAC (i, j) under 30 °C and 4 °C (n = 6 or 8 random fields per group). Arrows and arrowheads point to positive signals. Tumour growth rates of vehicle-fed and 15% glucose-treated melanoma (e) or PDAC (h) under 30 °C or 4 °C (n = 5 mice per group). k, Metabolomic heatmap analysis of glycolysis-related metabolites of vehicle- and HG-treated CRC tumours grown in WT or Ucp1−/− under 30 °C or 4 °C. l, Measurements of the amounts of glycolytic metabolites (n = 4 biological samples per group). m, qPCR analysis of Glut1 of vehicle- and HG-treated CRC under 30 °C or 4 °C (n = 3 samples per group). All scale bars, 50 μm. Data presented as mean ± s.e.m. Statistical analysis was performed using one-way ANOVA followed by Tukey multiple tests (a, m) or two-sided unpaired t-test (b, d, e, g, h, j, l). HG, high glucose (15% glucose); NS, not significant.
