Fig. 4: Metabolomic analysis of glycolysis and detection of PI3K signalling.
From: Brown-fat-mediated tumour suppression by cold-altered global metabolism
a, GSEA comparing the expression of carbohydrate metabolic process (top) and fatty acid metabolic process (bottom) of CRC samples exposed to 30 °C and 4 °C. n = 3 and 2 biological samples per group, respectively. b,c, Metabolomic heat-map analysis of glycolysis-related metabolites of BAT (b) and tumours (c) of CRC-tumour-bearing mice under 30 °C and 4 °C conditions. Each column represents a biological sample. n = 4 biological samples per group. d, Quantitative PCR (qPCR) analysis of Glut genes and glycolysis-related genes in CRC tumour tissues. n = 6 samples per group. e,f, Immunoblot analysis (e) and quantification (f) of non-phosphorylated and phosphorylated PI3K, AKT and mTOR in CRC tumours exposed to 30 °C and 4 °C. n = 4 biological samples per group. β-Actin was used for standardizing total protein loading levels. For d and f, data are mean ± s.e.m. Statistical analysis was performed using Wald tests (a) or two-sided unpaired t-tests (d and f); *P < 0.05, **P < 0.01, ***P < 0.001. Exact P values are provided in the Source Data.Source Data
