Extended Data Fig. 6: Evaluation of the cellular impact of extracting cytoplasm by Live-seq, relative to Fig. 3.

(a) Post-extraction viability as a function of the Live-seq-sampled volumes for ASPC (N = 33), IBA (N = 37) and RAW cells (N = 72). P = 0.44, 0.20, 0.18 for ASPC, IBA and RAW cells (two-sided Wilcoxon rank-sum test), respectively. The lower, centre and upper bounds of the box correspond to the first, second and third quartiles, respectively. The whiskers extend to the largest and smallest value no further than 1.5 inter-quartile range. (b) Cell volume distribution for ASPC (N = 277), IBA (N = 500), and RAW (N = 500) cell populations. (c) The correlations (R² of linear regression and P value (two-sided F-test)) between extracted cytoplasmic volume and either the number of detected genes (nGene) or total counts (nCount) for each indicated category are shown. (d) tSNE plots of RAW, IBA, and ASPC cells colored by extracted cytoplasmic volume. NA: data not available. Relative to Fig. 2b and Extended Data Fig. 2e, f.