Extended Data Fig. 4: RNAi perturbations of cortical condensates.
From: A condensate dynamic instability orchestrates actomyosin cortex activation
A-H, Left two panels, quantification of the efficacy of 24 h of RNAi via fluorescence imaging (right) and a quantification of the relative change in protein levels with respect to the non-RNAi control (left). Right two panels, WSP-1 and LifeAct fluorescent images of cortical condensates in the non-RNAi control (left) and after 24 h of RNAi (right) together with respective kymographs (bottom) determined along the white lines. A-D, 24h of RNAi directed against ARX-2, UNC-60, CAP-1 or COR-1 leads to stable large patches of WSP-1 and F-actin. In the case of COR-1, RNAi efficacy is reflected in a ~40% increase of F-actin at the cortex. E-H, 24hrs of RNAi directed against RHO-1, CYK-1, CDC42 or CHIN-1 does not appear to affect cortical condensates. In the case of RNAi directed against CDC42 and CHIN-1, RNAi efficacy is reflected in a ~ 40% and a ~ 15% increase in WSP-1. I, Dynamic cortical condensates persist for a strain with constitutively inactive NCK-1.
