Extended Data Fig. 2: Crystal structure of SHOC2 80-582. | Nature

Extended Data Fig. 2: Crystal structure of SHOC2 80-582.

From: Structure of the MRAS–SHOC2–PP1C phosphatase complex

Extended Data Fig. 2

a. Crystal structure of recombinant SHOC2 80-582 reveals a folded domain of 20 leucine rich repeats in excellent agreement with structure predictions. Both N and C terminal capping motifs are resolved, without density for the portion of the unstructured N terminus retained. LRR repeats are numbered. b. Alignment of the SHOC2 apo structure (grey) and SHOC2 from the ternary complex structure (pale green). The SHOC2 overall fold is identical, and the curvature of the solenoid shows only minor change to accommodate binding of the other complex members. c. The hydrogen bond network characteristic of the beta sheet traversing the concave face is disrupted between LRR 9 and 10, which is a component of slightly atypical LRR fold in the mid-range repeats of SHOC2.

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