Extended Data Table 2 Affinity of CRAF RBD to different RAS constructs by SPR

From: Structure of the MRAS–SHOC2–PP1C phosphatase complex

  1. Affinity of cRAF1-RBD (aa 31-151) to different avi-tagged and GppNHp -loaded MRAS constructs (immobilized on chip) was quantified by SPR to confirm similar affinity as to canonical RAS isoforms, validating the approach to set up respective TR-FRET assays. Individual results for N=2 independent experiments are given.
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