Extended Data Fig. 5: Different retrons inhibit growth in different environments.

a, STm and E. coli DE-COMM-4976 (Eco10), SC402 (Eco3), and REL606 (Eco1) (WT, ΔrnhA, ΔrcaT, or double mutant strains) were grown for 5-6 h at 37 °C in LB, serially diluted, and spotted on LB or LB pH 5.5 plates. Plates were incubated at indicated temperatures (n = 2 biological). b, Same strains as in panel a were grown anaerobically at 37 °C in LB for 24h in microtiter plates while measuring OD578. Here plotted the yield of mutant strains compared to the yield of the corresponding WT strain. n = 4 (2 biological/2 technical). c, Only RcaT-Sen2 and -Eco9 act as toxins when overexpressed alone. E. coli BW25113 strains carrying retron- or rcaT-encoding plasmids (Sen2, Eco9, Eco10, Eco3, and Eco1), were grown for 5-6 h at 37 °C in LB-spectinomycin, serially diluted, spotted on LB-spectinomycin plates with or without arabinose, and plates were incubated at 37 °C (n = 2 biological).