Fig. 5: Fos expression is associated with higher spatial map stability.

a, Example field of view, with a zoomed-in image (right). b, Representative place cells imaged on consecutive sessions. Top, jRGECO1a and fold-induction images. Bottom, average activity as a function of track position. Shading indicates the 1st and 99th percentiles for permuted activity relative to position. c, Example of place field stability for a subset of Fos-high (n = 289) and Fos-low (n = 230) place cells in one mouse. Black lines denote significant spatially binned activity in that portion of the track. A cell might appear more than once if it was induced on multiple sessions. Same ordering of cells across sessions. d, Schematic of stability calculation. For every pair of sessions, the correlation x between the spatially binned activity vectors is computed for each cell. Stability is the weighted mean of x in each bin, where weights w are proportional to the normalized activity of cells in that bin (Methods). e, Average stability maps for Fos-high and Fos-low populations. For each session-to-session comparison, Fos-high and Fos-low groups were determined on the basis of induction on session n. Data are shown as the mean ± s.e.m. n = 21, 17, 14 and 8 session-to-session comparisons, from left to right. f,g, Average stability in the peri-reward zone and no-reward zone of the track (zones indicated in h,i) across sessions. Data are shown as the mean ± s.e.m. n = 21, 17, 14, 8, 5 and 3 session-to-session comparisons, from left to right. h,i, Difference in stability between Fos-high and Fos-low (h) and Fos-KO (Cre⁺) and wild-type (WT) (i) cells as a function of track position. Black markers indicate bins where P < 0.05. ***P < 0.001 (h) and **P = 0.005 (i) for comparison of the mean stability difference in peri-reward and non-reward zones, two-sided bootstrap permutation test (1,000 shuffles). Data are shown as the mean ± s.e.m. n = 68 (h) and 114 (i) session-to-session comparisons.

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