Extended Data Fig. 3: Enrichment of Fos expression in navigation-related areas after virtual navigation.
From: Fos ensembles encode and shape stable spatial maps in the hippocampus
a) Schematic of the experiment. Nine mice were habituated to the apparatus and running on the ball for several days. On the day of the experiment, some mice were exposed to a virtual environment and spatial navigation task, while others remained in the dark as on previous days. Approximately 3.5 h after the start of behavior, brains were harvested, followed by staining for Fos expression. b) Representative histology images for Fos staining and corresponding atlas plate (reproduced, with permission, from Franklin and Paxinos79). Red boxes indicate approximate locations of enlarged image regions on the right. RSP, retrospenial; SSP, somatosensory; DG, dentate gyrus. c) Number of Fos-positive cells in 200 x 200 µm regions-of-interest (Methods). Error bars indicate mean ± s.e.m. n = 4 control mice and 4 exposed mice (2 with slices containing SSP). p = 0.046 (DG), p = 0.010 (CA1), p = 0.062 (RSP), p = 0.82 (SSP); two-sided t-test.
