Extended Data Fig. 4: Method for detecting significant calcium transients.
From: Fos ensembles encode and shape stable spatial maps in the hippocampus
a) Portion of a representative ∆F/F trace. b) The trace from (a) normalized and shown with upper (magenta) and lower (blue) σ thresholds for positive-going and negative-going transients. c) Binary matrix of significant transient frames for a subset of σ threshold levels. Black indicates significant transients at < 0.1% error rate. Note due to large number of frames in the example, some brief transients are not visible in the rendered image. d) Original ∆F/F trace with significant transient timepoints plotted in red. e) Significant transient ∆F/F trace with non-significant periods set to zero. f) Example error rate as a function of number of frames in the transient, plotted for different threshold levels. g) Representative traces for multiple cells with significant transients highlighted in red.
