Fig. 4: NPA competes with IAA and binds to a similar position in PIN1.
From: Structural insights into auxin recognition and efflux by Arabidopsis PIN1
a, ITC results for IAA binding to wild-type PIN1 at pH 7.0. Inset graphs show the original heat-change recordings. b, ITC results for NPA binding to wild-type PIN1 at pH 7.0. c, Characterization of auxin transport for wild-type (WT) and mutant PIN1 in HEK293F cells using the auxin efflux assay. d, Net IAA efflux with wild-type and mutant PIN1. Six independent experiments were performed for each construct in c,d. One-way ANOVA with Dunnett’s multiple comparisons test. ****P < 0.0001 for mutants versus wild type. Data are mean ± s.e.m.
