Extended Data Fig. 6: Caspase-6-mediated N cleavage modulates IFN signaling.
From: Coronaviruses exploit a host cysteine-aspartic protease for replication
a 293T cells were transfected with an IFN-β-Luc reporter plasmid, expression construct of MERS-CoV N, with or without poly(I:C). Cells were incubated for 24 h before harvesting for dual-luciferase reporter assays (n = 3). b 293T cells were transfected with an IFN-β-Luc reporter plasmid, expression constructs of MERS-CoV N or E and caspase-6 or caspase-3, with or without poly(I:C). Gene expression of IFN-β (n = 6), IFIT1 (n = 6), IFIT2 (n = 6), IFIT3 (n = 6), IFITM3 (n = 4), OAS1 (n = 4), and TRIM22 (n = 4) was quantified with RT-qPCR. c 293T cells were transfected with an IFN-β-Luc reporter plasmid, expression constructs of caspase-6 and MERS-CoV N, ORF4a, ORF4b, or M, with or without poly(I:C). Cells were incubated for 24 h before harvesting for dual-luciferase reporter assays (n = 3). d 293T cells were transfected with an IFN-β-Luc reporter plasmid, expression constructs of caspase-6 and two different amounts of MERS-CoV N, ORF4a, ORF4b, or M, with or without poly(I:C). Cells were incubated for 24 h before harvesting for dual-luciferase reporter assays (n = 3 for empty vector control and poly(I:C) only, n = 7 for all other groups). Data represented mean and standard deviations from the indicated number of biological repeats. Statistical significance between groups was determined with one way-ANOVA with Tukey’s post hoc tests.
