Extended Data Fig. 9: Caspase-6-cleaved N fragments of SARS-CoV-2.
From: Coronaviruses exploit a host cysteine-aspartic protease for replication
a Schematic of SARS-CoV-2 N mutants. b Caspase-6-mediated cleavage of SARS-CoV-2 N mutants was evaluated with Western blots. The experiment was repeated two times independently with similar results. c, d 293T (c) and Huh7 (d) cells were transfected with an IFN-β-Luc reporter plasmid, expression constructs of caspase-6 and SARS-CoV-2 N, N(1-215), N(216-419), with or without poly(I:C). Cells were incubated for 24 h before harvesting for dual-luciferase reporter assays. n = 5 for 293T cells. For Huh7 cells, n = 3 for the empty vector, poly(I:C) only, and poly(I:C)+caspase-6 groups; n = 6 for all other groups. e 293T cells were transfected with an IFN-β-Luc reporter plasmid, expression constructs of caspase-6, SARS-CoV-2 N, NSP13, NSP15, ORF6, ORF8, or ORF3b, with or without poly(I:C). Cells were incubated for 24 h before harvesting for dual-luciferase reporter assays (n = 3 for the empty vector and poly(I:C) only groups; n = 7 for all other groups). Data represented mean and standard deviations from the indicated number of biological repeats. Statistical significance between groups was determined with one way-ANOVA (c–e).
