Extended Data Fig. 10: Caspase-6-cleaved MERS-CoV N fragments colocalize with IRF3 in 293T and Huh7 cells.
From: Coronaviruses exploit a host cysteine-aspartic protease for replication
a 293T cells were transfected with expression constructs of IRF3, MERS-CoV N, N(1-241), or N(242-413), and poly(I:C). Cells were fixed at 16 h post transfection. Localization of N was detected with an in-house guinea pig anti-N immune serum and IRF3 was detected with a rabbit anti-HA antibody. Cell nuclei were identified with the DAPI stain. Bars in (a) represented 10 μm. b Huh7 cells were transfected with expression constructs of IRF3, MERS-CoV N, N(1-241), or N(242-413), and poly(I:C). Cells were fixed at 16 h post transfection. Localization of N was detected with an in-house guinea pig anti-N immune serum and IRF3 was detected with a rabbit anti-HA antibody. Cell nuclei were identified with the DAPI stain. Bars in (b) represented 10 μm. The experiments in (a, b) was repeated two times independently with similar results. c The Pearson correlation coefficient between MERS-CoV N fragments and IRF3 in Huh7 cells was quantified with the Zeiss ZEN software (n = 5). Data in (c) represented mean and standard deviations from the indicated number of biological repeats. Statistical significance between groups was determined with one way-ANOVA.
