Extended Data Fig. 9: Functional expression of the tabersonine/catharanthine and vindoline modules.
From: A microbial supply chain for production of the anti-cancer drug vinblastine
a. Construction of yeast strains for the functional expression of the tabersonine/catharanthine module (MIA-DC), the vindoline module (MIA-DE), and the tabersonine/catharanthine+vindoline modules (MIA-DJ); b. Production of catharanthine and tabersonine from fed 0.05 mM secologanin and 1 mM tryptamine; c. Production of vindoline from fed 0.05 mM tabersonine by strain MIA-DE expressing an additional copy of one or two genes from a centromeric plasmid; d. production of catharanthine, tabersonine and vindoline in MIA-DC and MIA-DJ strains fed with 0.1 mM secologanin and 1 mM tryptamine. e. Peak area of vindoline module intermediates in the cultivation of strain MIA-DE fed with 0.1 mM catharanthine and tabersonine. Data presented are mean ± s.d. (n = 3). * p-value < 0.05; ** p-value < 0.01; n.s., not significant. More statistical analysis is available in the source data file.
