Extended Data Fig. 12: Semi-synthesis of vinblastine by coupling of vindoline and catharanthine.
From: A microbial supply chain for production of the anti-cancer drug vinblastine
a. Semi-synthesis of vinblastine by chemical coupling of catharanthine and vindoline. Two methods were used for the coupling of vindoline and catharanthine, a photo-chemical coupling under near-UV light38 followed by reduction using NADH, and a chemical coupling using FeCl3, followed by reduction using NaBH42; b. Representative chromatograms of vinblastine standard (left), and vinblastine and anhydrovinblastine produced from the Fe(III)-based chemical coupling using yeast-produced catharanthine and vindoline (right). FMN: flavin mononucleotide.
