Extended Data Fig. 8: The TOR-FIE-PRC2-TR relay plays a central role in diverse developmental programs.
From: Glucose-driven TOR–FIE–PRC2 signalling controls plant development
a, TOR-FIE-PRC2 target genes. These genes are marked by H3K27me3 and upregulated in the SSTS/AAAA/fie mutant. b, Gene Ontology enrichment analysis of TOR-FIE-PRC2 target genes in biological process terms related to transcriptional regulation and development. Fisher’s exact test was used by the BiNGO to identify GO terms that are significantly over-represented with the compiled gene list. FDR, false discovery rate. The categories in biological process with fold enrichment > 2 and FDR < 10−10 were selected and presented. c, PRC2 target genes are transcription regulators upregulated in fie mutants (14d). UBQ10 or ACT2 transcripts served as an internal control for normalization. d, f, RT-qPCR analysis of TOR-FIE-PRC2 target genes in WT and tor-es. (d) In sugar-containing medium or (f) in sugar-free medium and without or with 25 mM glucose stimulation for 6 h after starvation (7 d). UBC21 transcripts served as an internal control for normalization. e, g, ChIP-qPCR analysis of H3K27me3 enrichment on TOR-FIE-PRC2 target genes in 7-day WT and tor-es. (e) in sugar-containing medium or (g) in sugar-free medium and without and with 25 mM glucose stimulation for 6 h after starvation (7 d). ChIP-qPCR data were normalized to percentage of input DNA. c-g, Data show mean ± s.d. from 3 biological replicates. Data were analysed by unpaired two-sided Student’s t test.
