Extended Data Fig. 1: TOR controls the global H3K27me3 level and development.
From: Glucose-driven TOR–FIE–PRC2 signalling controls plant development
a, TOR differentially regulates seedling biomass. WT seedlings treated with different concentrations of Torin2 or tor-es seedlings at 8 days after germination. (n = 6 seedlings). b, Distinct TOR activity thresholds regulate DNA replication. Quantification of EdU staining in roots (n = 5 seedlings). c, Metaplots showing ChIP-Rx-seq read density. H3K27me3 and H3K9me2 in 7-day WT and tor-es. The ChIP-seq data are normalized with an exogenous reference genome. The peak summits ±2 kb is shown. d, Genome browser view of H3K27me3 and H3K9me2 ChIP-seq read densities in WT and tor-es. The Arabidopsis genome region within Chr3:15900000..17800000 is shown. An enlarged view of a selected region is shown on the top. e, The H3K27me3 level in plants grown in sugar-containing medium. d, day. Values are the relative level of H3K27me3 compared with the corresponding H3 control, with immunoblot signals in day3 set as 1.0. Experiments were conducted in three biological repeats with similar results. Data in a, b show mean ± s.d., one-way ANOVA with Tukey’s multiple comparisons test.
