Extended Data Fig. 2: TOR controls the global H3K27me3 level.

a-d, TOR regulates global H3K27me3 levels. a, TOR but not S6K regulates global H3K27me3 levels. WT or S6K1-HA transgenic seedlings were treated with 10 μM of different inhibitors for 3 days. At 7 days, TOR activity was monitored by pS6K1(T449) and the band shift of pS6K1-HA. S6K activity was monitored by pRPS6(S237) and pRPS6(S240). The intensity of each immunoblot was quantified by ImageJ. Values are the relative level of H3K27me3 compared with the corresponding H3 control, with immunoblots in mock set as 1.0. b, Heatmap of H3K27me3 enrichment in plants with or without TOR inhibitor treatment. The colour scale indicates reference-adjusted RPM (RRPM) surrounding peak summit from the ChIP-Rx-seq data. c, Metaplots showing H3K27me3 ChIP-Rx-seq read density in plants with or without TOR inhibitor treatment. The ChIP-seq data are normalized with an exogenous reference genome. The peak summits ±2 kb is shown. d, Genome browser view of H3K27me3 ChIP-seq read densities. The Arabidopsis genome region within Chr3:15800000..18000000 is shown. e, Differential regulation of S6K and H3K27me3 in rap1 and lst8-1 mutants. The restoration of H3K27me3 was induced by 25 mM glucose for 6 h in 7-d sugar-starved seedlings. TOR-S6K activity was monitored by pRPS6(S237) and pRPS6(S240). The intensity of each immunoblot was quantified by ImageJ. Values for H3K27me3 are the relative level of H3K27me3 compared with the corresponding H3 control, with immunoblots in WT before glucose stimulation set as 1.0. Values for RPS6 phosphorylation are the relative level of pRPS6(S237) and pRPS6(S240) compared with the corresponding RPS6 control, with immunoblots in WT after glucose stimulation set as 1.0. Data in a and e are representatives of three biological replicates each.