Extended Data Fig. 1: Sequence alignment of the NiRAN domain reveals similarity to the pseudokinase selenoprotein O (SELO).

Multiple sequence alignment highlighting conserved kinase-like active site residues in the NiRAN domain among several CoVs, other selected Nidovirales (Arteri-, Mesoni- and Roniviruses) and the human SELO pseudokinase. Top: amino acid sequence surrounding the Lys-Glu ion pair. Bottom: and the amino acid sequence surrounding the active site and the “DFG” motif.