Extended Data Fig. 4: Literature-based prior network and signaling responses to ligand stimulation.

(A) Prior topology of core network connections based on the existing knowledge16,37,62. (B) Time courses of Trk and ERK activation (measured with phosphospecific antibodies, pTrk and ppERK) in TrkA and TrkB cells after stimulation with 100 ng/ml NGF or BDNF, respectively. GAPDH staining was used as loading control. For gel source data, see Supplementary Fig. 1. Representative blot of 3 biological replicates is shown.