Extended Data Fig. 6: Fate tracing of Evi1hi cells.
From: Independent origins of fetal liver haematopoietic stem and progenitor cells
a, Targeting strategy of the Evi1CreERT2 mouse. b, Fetal liver (FL) cellularity (Evi1+/+ mice, n = 7; Evi1CreERT2/+ mice, n = 10). c, HSC and progenitor fractions in Evi1CreERT2/+ (Evi1+/+ mice, n = 7; Evi1CreERT2/+ mice, n = 10; Evi1GFP/+ mice, n = 5). See Supplementary Discussion for HSC reduction in Evi1CreERT2 and Evi1GFP embryos. d, Labeling of Evi1-GFPhi cells by Evi1CreERT2. Evi1CreERT2 mice were crossed with Evi1GFPROSAtdTomato mice to obtain Evi1CreERT2/GFPROSAtdTomato embryos (tamoxifen at E10.5). n = 6. e, Whole-mount immunostaining analysis of E11.25 (tamoxifen at E10.5) Evi1CreERT2ROSAtdTomato embryos. Labeled endothelial cells and hematopoietic clusters are observed in the dorsal aorta. Scale bars, 100 μm. f, Whole-mount immunostaining analysis of E11.25 (tamoxifen at E10.5) Evi1CreERT2ROSAtdTomato embryos. In contrast to the vitelline and umbilical arteries, tdTomato+c-Kit+ cells are rarely observed in the yolk sac or fetal liver (orange arrows). Scale bars, 100 μm. g, Lineage tracing of Evi1+ cells. Evi1CreERT2ROSAtdTomato embryos were administered with tamoxifen at various stages (E8.5 and E9.5) and were analyzed at E14.5 (E8.5, n = 4; E9.5, n = 4). h, Whole-mount immunostaining analysis of E9.5 (tamoxifen at E8.5) Evi1CreERT2ROSAtdTomato embryo. Flat-shaped endothelial cells are labeled. Scale bar, 200 μm. All error bars represent means ± SD. Statistical analysis was performed two-sided unpaired Student’s t-test (b and c).
