Extended Data Fig. 3: Fate tracing of Hlf+ cells.
From: Independent origins of fetal liver haematopoietic stem and progenitor cells
a, Phenotypic comparison between E10.5 Hlf+c-Kit+ hematopoietic cluster cells and HlfCreERT2 -labeled cells in E11.5 fetal livers (tamoxifen at E9.75). b, Representative flow cytometry plots of HlfCreERT2-labeled cells in the AGM and yolk sac (red dots). c, Representative flow cytometry plots of tdTomato− fraction in the fetal liver and AGM of HlfCreERT2ROSAtdTomato embryos. d, Top, Schematic of tamoxifen treatment and analysis. Middle, Lineage tracing of Hlf+ cells. HlfCreERT2ROSAtdTomato embryos were administered with tamoxifen at various stages (E9.0–11.5) and were analyzed at E14.5. Each graph represents data from one litter. The highest frequency population is shaded (KL, gray; MPP, blue; HSC, orange). Bottom, Proportion of dominant-cell types.
