Extended Data Fig. 6: OTS964 blocks spliceosome before the first catalytic step of splicing.
From: CDK11 regulates pre-mRNA splicing by phosphorylation of SF3B1
a, b, Native gel analyses of spliceosome complex formation on radiolabelled AdML pre-mRNA in HeLa nuclear extracts treated with DMSO or indicated concentrations of OTS964 for indicated times. Identities of spliceosome complexes E, A, B and C are depicted on the side. -ATP corresponds to ATP-depleted nuclear extracts. c, Native gel analyses of spliceosome complexes upon treatment with OTS964 or indicated concentrations of control compounds or under condition of low concentration of MgCl2. See Extended Data Fig. 6a, b for legend.
