Extended Data Fig. 8: OTS964 and pladi B affect splicing via different mechanisms.
From: CDK11 regulates pre-mRNA splicing by phosphorylation of SF3B1
a, Immunoblot of proteins after treatment of WT or SF3B1 R1074H HCT116 cells with 186 nM pladi B for indicated times. b, Immunoblot of proteins after treatment of SF3B1 R1074H HCT116 cells with 50 nM OTS964 for indicated times. c, Immunoblot of proteins after treatment of CDK11 G579S HCT116 cells with 186 nM pladi B for indicated times. d, e, DNA gel-visualized RT-PCR analyses of splicing of Riok3 (d) and Ccnl1 (e) genes in WT, CDK11 G579S and SF3B1 R1074H HCT116 cells treated with control DMSO, 50 nM OTS964 or 186 nM pladi B for indicated times. Schema of unspliced and spliced products are depicted on the right and their ratio (spliced/unspliced) on bottom of gels. Markers on the left indicate size in nucleotides (nt) and DNA corresponds to genomic DNA control. f, Immunoblot analyses of HCT116 cell lysates treated with either 50 nM OTS964 for 4 h or untreated control. Pan P-RS = pan-phospho-RS specific antibody. Long and short correspond to long and short exposures of the film, respectively.
