Extended Data Fig. 1: Characterization of potential off-targets of OTS964.
From: CDK11 regulates pre-mRNA splicing by phosphorylation of SF3B1
a, b, Graphs show percentage of CDK9/CyclinT1 (a) and TOPK (b) kinase activities in the presence of increasing concentrations of OTS964 in IVKAs. Resulting IC50 from n = 2 replicates are shown in the graphs. c, Isothermal Titration Calorimetry (ITC) of TOPK and OTS964. d, Differential Scanning Fluorimetry (DSF) curve of TOPK and OTS964, staurosporine, DMSO and blank. e, f, Graphs show percentage of normalized NanoBRET ratio for CDK9/Cyclin T2 upon OTS964 or control dinaciclib treatment (n = 2 biologically independent replicates, a representative replicate is shown) (e) and for TYK2 upon OTS964 or control AT9283 treatment (n = 1 biological replicate) (f).
