Extended Data Fig. 2: OTS964 decreases bulk phosphorylation of all the CTD residues in a CDK11-dependent manner in cells.
From: CDK11 regulates pre-mRNA splicing by phosphorylation of SF3B1
a, Immunoblot of proteins after treatment of HCT116 cells with indicated concentrations of OTS964 for indicated times. b, Immunoblot of proteins after treatment of WT or CDK11 G579S HCT116 cells with 50 nM OTS964 or 200 nM flavopiridol for 4 h. c, d, Graphs show relative levels of mRNA of 14 histone genes in WT or CDK11 G579S HCT116 cells either treated with DMSO or with 50 nM OTS964 (c) or 200 nM flavopiridol (d) for 4 h. mRNA levels were normalized to Ppia mRNA expression and expression in DMSO was set as 1. n = 4 biologically independent experiments, error bars=SEM. e, Sulforhodamine B growth assays in WT and CDK11 G579S HCT116 cells treated with increasing concentrations of OTS964. n = 3 replicates of biologically independent experiments. f, Percentage of HCT116 WT and CDK11 G579S cells in individual cell cycle phases based on flowcytometry profiles of a representative replicate treated with the indicated concentrations of OTS964 for 16 h. n = 3 replicates of biologically independent experiments.
