Extended Data Fig. 7: (Related to Extended Data Fig. 1) FKBP12-FK506 interaction contributes to cellular accumulation of a fluorescence analog of FK506.
From: Brain-restricted mTOR inhibition with binary pharmacology
a, Structure of a fluorescent probe FK-TAMRA. b, FKBP12 knockdown decreases cellular retention of FK-TAMRA. Flow cytometry scatter plots shown are representative of three independent replicates, which are summarized in the bar graphs. Data are presented as mean values +/− SD with individual data points. Fold change is calculated as the ratio between means (sgRNA+/sgRNA−). See Supplementary Fig. 2 for gating strategy.
