Extended Data Fig. 1: FKBP12-Rapamycin interaction contributes to cellular accumulation of a fluorescent analog of RapaLink-1.
From: Brain-restricted mTOR inhibition with binary pharmacology
a, Illustration of the flow cytometry-based assay to assess cellular accumulation of TAMRA compounds. b, Structure of a fluorescent probe RapaTAMRA. c, FKBP12 knockdown decreases cellular retention of RapaTAMRA, but not TAMRA-PEG8-N3. Flow cytometry scatter plots shown are representative of three independent replicates, which are summarized in the bar graphs. Data are presented as mean values +/− SD with individual data points. Fold change is calculated as the ratio between means (sgRNA+/sgRNA−). See Supplementary Fig. 2 for gating strategy.
