Fig. 1: 3D reconstruction of the Sr35 resistosome.
From: A wheat resistosome defines common principles of immune receptor channels
a, Negative staining of purified Sr35 in complex with AvrSr35 (Sr35 resistosome). Star-shaped particles were enriched by affinity purification and size-exclusion chromatography. Monodisperse Sr35 resistosomes have an average size of approximately 24 nm. Scale bar, 100 nm. b, 2D classifications of the Sr35 resistosome particles from the cryo-EM sample. Particles show preferential orientations for bottom and top views. Fewer, but sufficient, particles are in side view. Scale bar, 20 nm. c, Cryo-EM density map with 3 Å resolution (top) and the finally refined structure model (bottom) of the Sr35 resistosome shown in three different orientations. AvrSr35 is coloured green and Sr35 domains are shown according to the colour codes in the inset panel.
