Extended Data Fig. 6: Cryo-EM data analysis of UDP-bound PsChs1.

a, Flowchart of cryo-EM data processing. b, FSC curves of UDP-bound PsChs1, including FSC between two independently refined half-maps generated by RELION (blue) and model-to-map FSC generated by Phenix (red). c, Angular distribution of the particles contributing to the final reconstruction of PsChs1. d, The map of UDP-bound PsChs1 is colored according to estimated local resolution. e, Sample maps of TM helices, N-terminal subdomains, and IF helices of UDP-bound PsChs1. f, EM density of UDP/Mg²⁺.