Extended Data Fig. 1: Sequence conservation among CHSs from oomycetes, fungi, and arthropods.

a, The structure of PsChs1 is colored according to sequence conservation among CHSs from different species. Residues are colored red, white and blue to indicate identical, similar and variable residues, respectively. The following sequences were used: PsChs1 (XP_009524159) (this study), PeChs1 (RMX65634) from Peronospora effusa, SmChs1 (ADE62520) from Saprolegnia monoica, AaChs1 (RHZ26641) from Aphanomyces astaci, ScChs1 (NP_014207), ScChs2 (NP_009594), and ScChs3 (NP_009579) from Saccharomyces cerevisiae (XP_011318411), CaChs2 (KHC63241) from Candida albicans, CiChs2 (KMP06892) from Coccidioides immitis, SbChs1 from Sporothrix brasiliensis (XP_040620288), TcChs1 from Tribolium castaneum (AAQ55059), PxChs1 (BAF47974) from Plutella xylostella, DmChsA (AAG22215) from Drosophila melanogaster, SfChs2 (AAS12599) from Spodoptera frugiperda, and TuChs1 (AFG28412) from Tetranychus urticae. b, Multiple sequence alignment of the catalytic domain of CHSs from different species was generated using Clustal X. Amino acids are highlighted by white (on red background), red and gray letters based on sequence identity, similarity and variability, respectively. Black boxes refer to conserved motifs, including motif 1 (TMYNE), motif 2 (DGR), motif 3 (KASKL), motif 4 (DVGT), motif 5 (QHFEY), motif 6 (VLPG), motif 7 (EDR), motif 8 (QRKRW), and motif 9 (SWG).