Extended Data Fig. 4: Phenotypic and functional analysis of LCMV-specific CD8+ T cells generated by PD-1, IL-2, and combination therapy during chronic infection.
From: PD-1 combination therapy with IL-2 modifies CD8+ T cell exhaustion program
LCMV chronically infected mice were either left untreated, or treated with anti-PD-L1 antibody alone, IL-2 therapy alone, or the combination therapy for 2 weeks. a, Representative FACS plots for co-expression of TIM3 and various phenotypic markers on DbGP33+ CD8+ T cells in spleens. b, c, One million splenocytes were cultured with recombinant mouse IL-12 and IL-18 (20 ng ml−1 each) for 5 h, then GolgiPlug was added, followed by culturing for 1 h. Note that no viral peptides were added to the culture. Cells were stained with surface markers including DbGP33-specific tetramer, fixed, and followed by intracellular staining of IFNγ. b, Representative FACS plots for co-staining of CD218a and IFNγ gated on DbGP33+ CD8+ T cells after the indicated treatments. c, Summary plots for the frequency of IFNγ+ cells in DbGP33+ CD8+ T cells. Results shown are representative flow plots from 2-7 experiments (a, b) or pooled from 7 experiments (c) with n = 2-5 per group in each experiment. Data are presented as mean and SD with p values (c). Statistical comparisons were performed using one-way ANOVA with Tukey’s multiple-comparison test (c). AF, Alexa Fluor; EF, eFluor; Untx, untreated.
