Extended Data Fig. 5: Identification of LCMV-specific CD8+ T cells generated after PD-1, IL-2, and combination therapy that produce cytokine after peptide stimulation.
From: PD-1 combination therapy with IL-2 modifies CD8+ T cell exhaustion program
LCMV chronically infected mice were either left untreated, or treated with anti-PD-L1 antibody alone, IL-2 therapy alone, or the combination therapy for 2 weeks. Spleen cells were stimulated with pools of LCMV-specific peptides for 5 h and analysed by intracellular staining for cytokine production. a, Representative UMAP with FlowSOM overlay of DbGP33+ CD8+ T cells isolated from spleens after the indicated treatments shows the distribution of cells in three clusters. b, Summary data for numbers of IFNγ+ LCMV-specific CD8+ T cells in the defined 3 clusters in the different treatment groups is shown. Results were pooled from 4 experiments with 2-3 mice per group in each experiment. Data are presented as mean and SEM (b) with p values. Statistical comparisons were performed using Kruskal-Wallis test with Dunn’s multiple-comparison test (d, e). Untx, untreated.
