Extended Data Fig. 12: Effect of IL-2(WT) versus IL-2(V) on LCMV-specific CD8+ T cells during chronic infection.
From: PD-1 combination therapy with IL-2 modifies CD8+ T cell exhaustion program
a, Experimental design for data in panels b and c. Mice chronically infected with LCMV (> 40 days post infection) were untreated or treated with IL-2(WT) for 5 days, and CD25 expression was checked on PD-1negative and PD-1+ CD8+ T cells in the spleen. b, Representative FACS plots of CD25 expression. c, Summary plots of CD25 expression after IL-2(WT) and IL-2(V) treatments. d, Experimental design for data in panels e and f. Mice chronically infected with LCMV were untreated, treated with IL-2(WT) or treated with IL-2(V) for 6 days. Expression of IL-2 receptors (CD25, CD122, and CD132) on LCMV-specific CD8+ T cells in the spleen were examined. e, f, Representative histograms (e) and summary plots (f) of expression of IL-2 receptors on DbGP33+ CD8+ T cells after indicated treatments. Results were pooled from 2 experiments with 2-3 mice per group in each experiment. Data are presented as mean and SD (c, f) with p values. Statistical comparisons were performed using one-way ANOVA with Tukey’s multiple-comparison test. Untx, untreated.
