Extended Data Fig. 5: Effects of CellREADR on targeted mRNA.
From: Programmable RNA sensing for cell monitoring and manipulation
a, Quantitative PCR showing that CellREADR-mediated sesRNA expression did not impact the expression levels of targeted RNAs. Error bars are mean values ± s.e.m. n = 3 biological replicates performed. Ordinary one-way ANOVA followed by Dunnett's multiple comparisons with the mean of control was used for analysis. All the P values are indicated. b, Base-pairing of EEF1A1 mRNA and sesRNAEEF1A1-CDS. EEF1A1 mRNA or sesRNA was represented in blue and red, respectively. Peptide translated from EEF1A1 mRNA was highlighted in brown. Targeted region of EEF1A1 mRNA was analyzed by RNAseq. c, The ratios of A-to-G changes in EEF1A1 mRNA at each adenosine position was quantified and shown in heatmap in both sesRNAEEF1A1-CDS and sesRNACtrl samples. Two adenosines (A107 and A115) showed higher rate of A-to-G editing (c). Off-target editing of two sensitive adenosines can induce potential amino acid change (underlined in b). d, Base-pairing of PCNA mRNA and sesRNAPCNA. PCNA mRNA or sesRNA was represented in blue and red, respectively. Peptide translated from PCNA mRNA was highlighted in brown. Targeted region of PCNA mRNA was analyzed by RNAseq. e, The ratios of A-to-G changes in PCNA mRNA at each adenosine position was quantified and shown in heatmap in both sesRNAPCNA and sesRNACtrl samples. Three adenosines (A21, A29 and A129) showed higher rate of A-to-G editing. Off-target editing of three sensitive adenosines can induce potential amino acid change (underlined in d).
