Extended Data Fig. 9: Visual schematic illustrating the main results.

DNA-sequencing of multiple glands from up to four tumour regions allowed elucidation of tumour evolutionary history and selection inference (left; phylogenetic tree). Matched RNA-sequencing found few genes with heritable expression patterns (middle; bars represent expression level), with the expression of most genes not detectably related to tumour evolutionary history (right; bars represent expression level). Most putative subclonal driver mutations were found to not be under selection, while transcriptomic differences could be found between subclones that were under positive selection. Sample names and bars are coloured according to region-of-origin.