Fig. 1: The multipass translocon is distinguished by three obligate heterocomplexes.
From: Substrate-driven assembly of a translocon for multipass membrane proteins
a, Experimental strategy. Nuclease-treated membranes from wild-type or stably integrated Flag-tagged (TMCO1, Nicalin (also known as NCLN) and CCDC47) HEK293 cells were digitonin-solubilized, immunoprecipitated and sedimented through a sucrose cushion to isolate the ribosome-bound and ribosome-free fractions for analysis. b, Analysis of input (I), ribosome-bound (pellet (P)) and ribosome-free (supernatant (S)) fractions by SDS–PAGE and immunoblotting. uL22 and STT3A are used here as markers for the ribosome and OST, respectively. IP, immunoprecipitation. c, Whole-cell lysates from the indicated wild-type and knockout HEK293 cell lines were analysed by SDS–PAGE and immunoblotting. d, Subunit organization and key architectural features of the compositionally distinct multipass, core and secretory translocons, viewed from the cytosol. Source data for all gels can be found in Supplementary Fig. 1.
