Fig. 3: Internal loop translocation at the secretory translocon.
From: Substrate-driven assembly of a translocon for multipass membrane proteins
a, The template used to generate truncated, Flag-tagged EAAT1 constructs, as in Fig. 2a. b, Stalled, Flag-tagged EAAT1 constructs truncated at the indicated positions were analysed as in Fig. 2b. The appearance of additional EAAT1 bands in later intermediates (EAAT1(268) onwards) is due to glycosylation in the TM3–TM4 luminal loop. c, Stalled, Flag-tagged EAAT1 constructs truncated at the indicated positions were translated in RRL in the presence of wild-type and double-knockout (TMCO1/CCDC47 (ΔTC)) rough microsomes, and analysed as in b. d, Diagram of translocon composition at different stages of EAAT1 synthesis, based on data in b,c. Glycosylation of the EAAT1 acceptor site(s) is indicated.
