Extended Data Fig. 1: Three obligate complexes of the multipass translocon.
From: Substrate-driven assembly of a translocon for multipass membrane proteins
a, Whole cell lysates from siRNA depleted HEK293 cells were analyzed by SDS-PAGE and immunoblotting; ‘Ctrl’ is a non-targeting control siRNA. b, Nomenclature, subunit topology and number of transmembrane domains for three obligate heterocomplexes of the multipass translocon. Subunits that directly contact the ribosome in the assembled state are indicated with an asterisk. Within the translocon, CCDC47 and TMEM147 contact different regions of Sec61, while interactions between the PAT, GEL and BOS complexes are limited to small portions of TMCO1 that contact CCDC47, TMEM147 and Nicalin. c, Distribution of the multipass translocon components in eukaryotes (OrthoDB v10.1).
