Extended Data Fig. 2: Translocon dynamics during TRAM2 synthesis.
From: Substrate-driven assembly of a translocon for multipass membrane proteins
a, Template used to generate truncated, Flag-tagged TRAM2 constructs, as in Fig. 2a. b, Stalled, Flag-tagged TRAM2 constructs truncated at the indicated positions were analyzed as in Fig. 2b. Note that the 140-mer intermediate (and beyond) is glycosylated at position 55. c, Stalled, Flag-tagged TRAM2 constructs truncated at the indicated positions were translated in RRL in the presence of WT and double knockout (TMCO1/CCDC47, ‘ΔTC’) rough microsomes, and analyzed as in (b).
