Extended Data Fig. 3: Translocon dynamics during KDELR1 synthesis. | Nature

Extended Data Fig. 3: Translocon dynamics during KDELR1 synthesis.

From: Substrate-driven assembly of a translocon for multipass membrane proteins

Extended Data Fig. 3

a, Template used to generate truncated, Flag-tagged KDELR1 constructs, as in Fig. 2a. b, Stalled, Flag-tagged KDELR1 constructs truncated at the indicated positions were analyzed as in Fig. 2b. c, Stalled, Flag-tagged KDELR1 constructs truncated at the indicated positions were translated in RRL in the presence of WT and double knockout (TMCO1/CCDC47, ‘ΔTC’) rough microsomes, and analyzed as in (b).

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