Extended Data Fig. 10: Comparison of apo-EnT1.3 (PDB: 7ZP6) and product-bound EnT1.3ΔC310-314 (PDB: 7ZP7).
From: A designed photoenzyme for enantioselective [2+2] cycloadditions
a, EnT1.3 (shown as a grey cartoon) has a hydrogen-bonding network formed of E225, R196 and D149 (shown as dashed black lines). The side chains of key residues are shown as atom-coloured sticks with grey carbons. BpA173 is shown as atom-coloured sticks and semitransparent CPK spheres with green carbons. b, The structure of EnT1.3ΔC310-314 (shown as a blue cartoon) in complex with the product (-)-1a (shown as atom-coloured sticks with purple carbons). The side chains of key residues are shown as atom-coloured sticks with blue carbons. BpA173 is shown as atom-coloured sticks and semitransparent CPK spheres with green carbons. When in complex with product, R196 lies in a different conformation to that shown in the structure of apo-EnT1.3, instead forming a hydrogen bond with Q195. Q195 and Y121 form close hydrogen bonds to (-)-1a.
