Extended Data Fig. 1: BpA positioning in the DA_20_00 scaffold.
From: A designed photoenzyme for enantioselective [2+2] cycloadditions
a, Positions within DA_20_00 selected to incorporate the photosensitizer BpA are shown as green CPK spheres at the C-alpha. b, Bar chart showing reaction conversions and selectivities achieved by DA_20_00 variants with BpA installed at the specified positions. Reaction conditions: 15 μM catalyst, 400 μM 1, 30 min irradiation (10 s on/off pulse) at 365 nm, 4 °C, 100 μl PBS (pH 7.4) with 5% DMSO as a cosolvent in a 96-well plate. e.e. is given for the major product, (-)-1a. Error bars represent the standard deviation of measurements made in triplicate.
