Extended Data Fig. 4: Reaction time courses of EnT1.0 and EnT1.3.
From: A designed photoenzyme for enantioselective [2+2] cycloadditions
Reaction conditions: 15 μM catalyst, 400 μM 1, 10 s on/off pulse at 365 nm, 4 °C, 1 ml PBS (pH 7.4) with 5% DMSO as a cosolvent in 2-ml glass vials. At each time point, 100 μl of reaction mix was quenched with one volume of MeCN and analysed by UPLC. Reaction conversion of 1 to 1a and 1b is given over 5 min. The gradients of a linear fit to the data are given for each variant. A table providing conversion data is given in Supplementary Table S2. Error bars represent the standard deviation of measurements made in triplicate (error bars are not shown when they are smaller than the data-point marker).
